Mac OS X 10.4 or later
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Features and Highlights
Create and arrange image frames in the filmstrip view to piece together animations; export animations as animated GIFs, QuickTime movies, or even spritesheets!
Leverage the app's wide array of tools when composing pixel art: Pencil, Eraser, Eyedropper, Zoom, Rectangular Selection, Magic Wand, Lasso, Move, Fill, Line, Rectangle, and Ellipse.
Take advantage of the flexibility layers provide when working with complex pixel art.
Add and organize commonly-used colors into palettes.
Define custom patterns to use while drawing with the pencil, line, or eraser tool (instead of a single pixel).
Pick a flat, striped or checkered background (or even a custom image) to be drawn beneath the canvas.
Pixen supports pressure-sensitive drawing tablets and maps pressure to color opacity.
Work with all your favorite tools in full screen mode on OS X Lion.
Note: Pixen is now a paid app in the Mac App Store. Requires 64-bit processor.